Bio1

Biological Matrices

Development of analytical methods for the extraction and clean-up of biologically active pharmaceuticals (steroid hormones, fluoroquinolones, β-lactams) in human plasma and urine.

Description

Development of analytical methods for the extraction and clean-up of biologically active pharmaceuticals (steroid hormones, fluoroquinolones, β-lactams) in human plasma and urine. The main tasks are:

  • Preparation and application of polymerized high internal phase emulsions (polyHIPEs) and molecular imprinting polyHIPEs for packed-cartridge micro-solid-phase extraction (µ-SPE)
  • Design and application of various types of carbonaceous materials from renewable sources (e.g., humic acids and bio-wastes) for magnetic SPE and pipette-tip SPE

Publications

Valentina Quarta , Francesca Merlo , Chiara Milanese , Antonella Profumo , Andrea Speltini

Biochar-based “in-vial” stir bar sorptive microextraction for a greener determination of steroid hormones in saliva

Published in:  Journal of Chromatography Open 9 (2026) 100287

Speltini Andrea
Profumo Antonella
Merlo Francesca
Quarta Valentina

F. Merlo, F. Colucci, G. De Soricellis, F. Rinaldi, E. Calleri, A. Profumo, A. Speltini

Carbon nanotubes in high internal phase emulsion polymer composite for packed-cartridge micro-solid-phase extraction of fluoroquinolones in urine

Published in:  Advances in Sample Preparation 9 (2024) 100103

F. Merlo, P. Bianchini, F. Maraschi, A. Speltini, A. Profumo

Pipette-tip μSPE coupled with HPLC-MS/MS for trace determination of fluoroquinolones in human urine

Published in:  Talanta Open 8 (2023) 100271

L. Ferrari, A. Speltini

Neonicotinoids: an overview of the newest sample preparation procedures of environmental, biological and food matrices

Published in:  Adv. Sample Prep. 8 (2023) 100094

F. Merlo, J. Montagna, F. Maraschi, A. Profumo, F. Baldanti, A. Speltini

A versatile method for multiclass determination of β-lactam drugs in urine by solid-phase extraction followed by HILIC-UV

Published in:  Journal of Chromatography Open 2 (2022) 100048

A. Speltini, F. Merlo, F. Maraschi, G. Marrubini, A. Faravelli, A. Profumo

Magnetic Micro-Solid-Phase Extraction Using a Novel Carbon-Based Composite Coupled with HPLC–MS/MS for Steroid Multiclass Determination in Human Plasma

Published in:  Molecules 26 (2021) 2061

A. Speltini, F. Merlo, F. Maraschi, L. Villani, A. Profumo

HA-C@silica sorbent for simultaneous extraction and clean-up of steroids in human plasma followed by HPLC-MS/MS multiclass determination

Published in:  Talanta 221 (2021) 121496

G. Marrubini, S. Dugheri, G. Cappelli, G. Arcangeli, N. Muzzi, P. Appelblad, C. Melzi, A. Speltini

Experimental designs for solid-phase microextraction method development in bioanalysis: a review

Published in:  Anal. Chim. Acta 1119 (2020) 77-100